The -1355G/C Polymorphism of Ferroportin (FPN1) Gene Among Adolescent Girls With Iron Deficiency Anemia

Sri, Wahyuni and Siti, Wahyuningsih and Arta, Farmawati and Ahmad, Hamim Sadewa (2015) The -1355G/C Polymorphism of Ferroportin (FPN1) Gene Among Adolescent Girls With Iron Deficiency Anemia. AICSTS 2015. pp. 96-104. ISSN 2477-1899


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Ferroportin is a cellular iron exporter protein that has been identified in human and plays an important role for iron homeostasis in tissues. The −1355G/C polymorphism in ferroportin (FPN1) gene promoter region leads to increase in ferroportin expression and iron export, decreases in hemoglobin and serum ferritin levels that manifest as iron deficiency anemia (IDA). The aim of this study was to investigate the FPN1 −1355G/C polymorphism as a risk factor of IDA. Polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP) with Bfa1 restriction enzyme was used to determine the −1355G/C polymorphism among 20 adolescent girls with anemia (cases) and 52 healthy controls. The blood samples were collected and analyzed for complete blood count. Serum ferritin levels were measured by ELISA. All of the data was obtained by statistically analyzed by the chi-square test or Fisher’s exact test, independent sample ttest and Mann-Whitney Test. Genotypes distribution showed no significant differences between IDA group (CC 38.5%, CG 53.8%, and GG 7.7%) and control group (CC 36.5%, 55.8% CG, 7.7%). The odds ratio (OR) of C allele having IDA was 2.036 (95% CI, 0.518-7.995, p=0.249). Subjects carrying the C allele in the IDA group had lower serum ferritin levels than the non-IDA group (p=0.000). This study suggests that the C allele of FPN1 −1355G/C genetic polymorphism is a risk factor for iron deficiency anemia among adolescent girls. Key words: Polymorphism, iron deficiency anemia, ferritin serum levels, ferroportin, FPN1 −1355G/C

Item Type: Article
Subjects: R Medicine > R Medicine (General)
R Medicine > RZ Other systems of medicine
Depositing User: dr. Sri Wahyuni, M.Sc.
Date Deposited: 05 Dec 2017 09:18
Last Modified: 05 Dec 2017 09:18

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