The Difference in the Antimicrobial effect of Katuk Leaf Extract (Sauropus Androgynus (l.) Merr.) Concentration against Escherichia Coli

Husna, Cut Asmaul and Al-Muqsith, Al-Muqsith and Soya Loviana, Hasibuan (2018) The Difference in the Antimicrobial effect of Katuk Leaf Extract (Sauropus Androgynus (l.) Merr.) Concentration against Escherichia Coli. Proceedings of MICoMS 2017 (Emerald Reach Proceedings Series, Volume 1), 1. p. 361366. ISSN 978-1-78756-793-1

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Abstract

Abstract Purpose – The aim of this study is to determine the differences in the antimicrobial activity of katuk leaf (Sauropus androgynus (L.) Merr) against Escherichia coli. Design/Methodology/Approach – The method used in this study was experimental posttest using a control group design. Analysis of the effect of katuk leaf was performed in the dilution method with 20%, 40%, 60%, 80%, and 100% concentration. The data were analyzed using one-way ANOVA test (α = 0.05) and was then tested using the least significant difference (LSD) test. Findings – Bacterial colony counting that used total plant count found the average of E. coli amount at 20% of concentration (526.820 CFU/ml), 40% of concentration (449.380 CFU/ml), concentration of 60% (255.710 CFU/ml), concentration of 80% (194.110 CFU/ml), and at concentration 100% (168.600 CFU/ml). This study concluded that the katuk leaf extract at 20%, 40%, 60%, 80%, and 100% of concentration had antimicroba effect with significant influence. The 100% of concentration had the most significant effect compared with the other concentrations. Research Limitations/Implications – Katuk leaf could be used as one of the alternative herbal choices that has a compound antimicrobial effect. Originality/Value – This study increases the theoretical understanding of the difference of antimicrobial effectivity of katuk leaf extract (S. Androgynus (L.) Merr.) concentration against E. coli Keywords: Escherichia coli , antimicrobial, extract, katuk leaf

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